So now you know the difference between ethanol and isopropanol precipitation, and when to use each method. Good luck with your DNA precipitations! Has this helped you? Then please share with your network. Hi in my script it is nowhere mentioned that we are supposed to use salt. Does it still work without adding salt or is it a mistake in the script?
Could you please help me out? Thanks for this clear explanation. It helps a lot the students who discover the beautiful world of molecular biology! Facebook Twitter LinkedIn More. Written by Dr Nick Oswald. Incubate on ice for 15 minutes. In case of small DNA fragments or high dilutions overnight incubation gives best results. Discard supernatant by decanting or pipetting, being careful not to throw out DNA pellet which may or may not be visible. Add 0. Calculate after addition of sodium acetate.
Room temperature isopropanol minimizes coprecipitation of salt. Discard supernatant by decanting, being careful not to throw out DNA pellet which may or may not be visible. Isopropanol precipitated pellets are often difficult to see and loosely attached.
Mark outside of tube before centrifugation for easy identification. Topics: Molecular Biology. This assay is suitable for the simple and rapid estimation of protein concentration.
This removes co-precipitated salt and replaces the isopropanol with the more volatile ethanol, making the DNA easier to redissolve. Carefully decant the supernatant without disturbing the pellet. Air-dry the pellet for 5—20 min depending on the size of the pellet.
Redissolve the DNA in a suitable buffer.
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